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验证数据展示

  • IP experiment of HEK-293 using 21599-1-AP

    IP experiment of HEK-293 using 21599-1-AP

    IP result of anti-DNA2 (IP:21599-1-AP, 4ug; Detection:21599-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • IHC staining of human liver cancer using 21599-1-AP

    IHC staining of human liver cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 21599-1-AP

    IHC staining of human liver cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNA2 Polyclonal antibody

DNA2 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 21599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IP, IHC, ELISA

别名

DNA2, DNA2L, KIAA0083

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21599-1-AP targets DNA2 in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DNA2 fusion protein Ag16206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA replication helicase 2 homolog (yeast)
别名 DNA2, DNA2L, KIAA0083
计算分子量 1060 aa, 120 kDa
观测分子量 125 kDa
GenBank蛋白编号BC111740
基因名称 DNA2
Gene ID (NCBI) 1763
RRIDAB_2878890
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

实验方案

Product Specific Protocols
IHC protocol for DNA2 antibody 21599-1-APDownload protocol
IP protocol for DNA2 antibody 21599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols