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验证数据展示

  • WB analysis of mouse cerebellum using 66891-2-Ig

    WB analysis of mouse cerebellum using 66891-2-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 66891-2-Ig

    IF Staining of Jurkat using 66891-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DMXL2 antibody (66891-2-Ig, Clone: 1F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

DMXL2 Monoclonal antibody

DMXL2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66891-2-Ig

产品说明书

CloneNo. 1F9A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, Jurkat cells, rat brain tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DMXL2 Ready-To-Use IHC Kit. DMXL2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66891-2-Ig targets DMXL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag19861

Product name: Recombinant human DMXL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 573-922 aa of BC140781

Sequence: DSHHTLLHQEGMSVGSPHGSQPHSRSHSTHMNILAPTVMMISKHIDGSLNQWAVTFADKSAFTTVLTVSHKFRYCGHRFHLNDLACHSVLPLLLTSSHHNALLTPELDCQWDSDNKLSRLMDPVKHIKGSSKQPLRNAATRTFHDPNAIYSELILWRVDPIGPLSYTGGVSELARINSLHTSAFSNVAWLPTLIPSYCLGTYCNSASACFVASDGKNLRLYQAVVDARKLLDELSDPESSKLIGEVFNIVSQQSTARPGCIIELDAITNQCGSNTQLLHVFQEDFIIGYKPHKEDMEKKETEIFFQPSQGYRPPPFSEKFFLVVIEKDSNNNSILHMWHLHLKSVQACLG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Dmx-like 2
别名 Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2
计算分子量 3036 aa, 340 kDa
观测分子量 340 kDa
GenBank蛋白编号BC140781
基因名称 DMXL2
Gene ID (NCBI) 23312
RRIDAB_2882221
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles.

实验方案

Product Specific Protocols
IF protocol for DMXL2 antibody 66891-2-IgDownload protocol
WB protocol for DMXL2 antibody 66891-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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