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DMWD Polyclonal antibody

DMWD Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16070-1-AP

Print datasheet

Synonyms

D19S593E, DM9, DMR N9, DMRN9, DMWD, gene59, Protein 59, Protein DMR N9

验证数据展示

  • WB analysis using 16070-1-AP

    WB analysis using 16070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16070-1-AP (DMWD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16070-1-AP

    IHC staining of mouse brain using 16070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16070-1-AP

    IHC staining of mouse brain using 16070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16070-1-AP targets DMWD in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, rat, mouse
Immunogen DMWD fusion protein Ag9007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dystrophia myotonica, WD repeat containing
Synonyms D19S593E, DM9, DMR N9, DMRN9, DMWD, gene59, Protein 59, Protein DMR N9
Calculated Molecular Weight 674 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC019266
Gene Symbol DMWD
Gene ID (NCBI) 1762
RRIDAB_2230476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09019
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DMWD antibody 16070-1-APDownload protocol
IHC protocol for DMWD antibody 16070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols