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验证数据展示

  • WB analysis using 16070-1-AP

    WB analysis using 16070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16070-1-AP (DMWD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16070-1-AP

    IHC staining of mouse brain using 16070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16070-1-AP

    IHC staining of mouse brain using 16070-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DMWD Polyclonal antibody

DMWD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16070-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA

别名

D19S593E, DM9, DMR N9, DMRN9, DMWD, gene59, Protein 59, Protein DMR N9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16070-1-AP targets DMWD in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat, mouse
免疫原

CatNo: Ag9007

Product name: Recombinant human DMWD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-334 aa of BC019266

Sequence: MLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAATAAGADGERSGEEEEEEPEAAGTGSAGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPAASSSRGGEPGPGPLPRSLSRSNSLPHPAGGGKAGGPGVAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGSGSGGEKPSGPVPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGNSPSGTVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dystrophia myotonica, WD repeat containing
别名 D19S593E, DM9, DMR N9, DMRN9, DMWD, gene59, Protein 59, Protein DMR N9
计算分子量 674 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC019266
基因名称 DMWD
Gene ID (NCBI) 1762
RRIDAB_2230476
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ09019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DMWD antibody 16070-1-APDownload protocol
WB protocol for DMWD antibody 16070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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