验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse ovary tissue |
| Positive IP detected in | mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag9242 Product name: Recombinant human DMKN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-476 aa of BC011886 Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dermokine |
| 别名 | Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411 |
| 计算分子量 | 476 aa, 47 kDa |
| 观测分子量 | 48-55 kDa |
| GenBank蛋白编号 | BC011886 |
| 基因名称 | DMKN |
| Gene ID (NCBI) | 93099 |
| RRID | AB_10596473 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6E0U4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for DMKN antibody 16252-1-AP | Download protocol |
| WB protocol for DMKN antibody 16252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome. | ||
Mol Cell Proteomics Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions. |
