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验证数据展示

  • WB analysis using 16252-1-AP

    WB analysis using 16252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 16252-1-AP

    IP experiment of mouse lung using 16252-1-AP

    IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

DMKN Polyclonal antibody

DMKN Polyclonal Antibody for WB, IP, ELISA
Cat No. 16252-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse ovary tissue
Positive IP detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 DMKN fusion protein Ag9242 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dermokine
别名 Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411
计算分子量 476 aa, 47 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC011886
基因名称 DMKN
Gene ID (NCBI) 93099
RRIDAB_10596473
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6E0U4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

实验方案

Product Specific Protocols
WB protocol for DMKN antibody 16252-1-APDownload protocol
IP protocol for DMKN antibody 16252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.

Authors - Tholen Stefan S
View Article

Mol Cell Proteomics

Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.

Authors - Pascal Schlage
View Article