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验证数据展示

  • WB analysis using 16252-1-AP

    WB analysis using 16252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 16252-1-AP

    IP experiment of mouse lung using 16252-1-AP

    IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

DMKN Polyclonal antibody

DMKN Polyclonal Antibody for WB, IP, ELISA
Cat No. 16252-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse ovary tissue
Positive IP detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag9242

Product name: Recombinant human DMKN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-476 aa of BC011886

Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dermokine
别名 Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411
计算分子量 476 aa, 47 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC011886
基因名称 DMKN
Gene ID (NCBI) 93099
RRIDAB_10596473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6E0U4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

实验方案

Product Specific Protocols
WB protocol for DMKN antibody 16252-1-APDownload protocol
IP protocol for DMKN antibody 16252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.

Authors - Tholen Stefan S
View Article

Mol Cell Proteomics

Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.

Authors - Pascal Schlage
View Article