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验证数据展示

  • WB analysis using 24813-1-AP

    WB analysis using 24813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24813-1-AP

    IF Staining of HepG2 using 24813-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 24813-1-AP

    FC experiment of HepG2 using 24813-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human liver cancer using 24813-1-AP

    IHC staining of human liver cancer using 24813-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 24813-1-AP

    IHC staining of human liver cancer using 24813-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens).

DMGDH Polyclonal antibody

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 24813-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

别名

dimethylglycine dehydrogenase, DMGDHD, EC:1.5.8.4, M2GD, ME2GLYDH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24813-1-AP targets DMGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag20529

Product name: Recombinant human DMGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 284-613 aa of BC022388

Sequence: ARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKDQNSCFAHFKEENGWVSRWAIRPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dimethylglycine dehydrogenase
别名 dimethylglycine dehydrogenase, DMGDHD, EC:1.5.8.4, M2GD, ME2GLYDH
计算分子量 866 aa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC022388
基因名称 DMGDH
Gene ID (NCBI) 29958
RRIDAB_2879739
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.

实验方案

Product Specific Protocols
FC protocol for DMGDH antibody 24813-1-APDownload protocol
IF protocol for DMGDH antibody 24813-1-APDownload protocol
IHC protocol for DMGDH antibody 24813-1-APDownload protocol
WB protocol for DMGDH antibody 24813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
View Article
humanWB,IHC

Oncotarget

Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo.

Authors - Gang Liu
View Article
humanWB

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
View Article
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