验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells, L02 cells, SMMC-7721 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
33988-1-AP targets DMBX1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag41356 Product name: Recombinant human DMBX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 150-250 aa of BC156881 Sequence: EGKAEAPTPDTQLDTEQPPRLPGSDPPAELHLSLSEQSASESAPEDQPDREEDPRAGAEDPKAEKSPGADSKGLGCKRGSPKADSPGSLTITPVAPGGGLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diencephalon/mesencephalon homeobox 1 |
| 别名 | Diencephalon/mesencephalon homeobox protein 1, MBX, Orthodenticle homolog 3, OTX3, Paired-like homeobox protein DMBX1 |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC156881 |
| 基因名称 | DMBX1 |
| Gene ID (NCBI) | 127343 |
| RRID | AB_3743134 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8NFW5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DMBX1 is a homeobox transcription factor with tightly regulated expression in the developing diencephalon and mesencephalon, where it governs neuronal differentiation and regional patterning. Acting through sequence-specific DNA binding, DMBX1 orchestrates developmental gene networks essential for central nervous system formation. Aberrant regulation of DMBX1 is associated with neurodevelopmental abnormalities and neural-derived cancers, underscoring its importance in neural lineage specification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DMBX1 antibody 33988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
