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验证数据展示

  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DMBT1 Polyclonal antibody

DMBT1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27069-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF-P, ELISA

别名

muclin, Hensin, Gp-340, GP340, Gp 340

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman stomach cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27069-1-AP targets DMBT1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16229

Product name: Recombinant human DMBT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1313-1662 aa of BC153299

Sequence: IEVFDGPYRSSPLIARVCDGARGSFTSSSNFMSIRFISDHSITRRGFRAEYYSSPSNDSTNLLCLPNHMQASVSRSYLQSLGFSASDLVISTWNGYYECRPQITPNLVIFTIPYSGCGTFKQADNDTIDYSNFLTAAVSGGIIKRRTDLRIHVSCRMLQNTWVDTMYIANDTIHVANNTIQVEEVQYGNFDVNISFYTSSSFLYPVTSRPYYVDLNQDLYVQAEILHSDAVLTLFVDTCVASPYSNDFTSLTYDLIRSGCVRDDTYGPYSSPSLRIARFRFRAFHFLNRFPSVYLRCKMVVCRAYDPSSRCYRGCVSRSKRDVGSYQEKVDVVLGPIQLQTPPRREEEPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deleted in malignant brain tumors 1
别名 muclin, Hensin, Gp-340, GP340, Gp 340
计算分子量 2413 aa, 261 kDa
GenBank蛋白编号BC153299
基因名称 DMBT1
Gene ID (NCBI) 1755
RRIDAB_2880741
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deleted in malignant brain tumors 1 (DMBT1), also known as the salivary scavenger and agglutinin (SALSA), salivary agglutinin (SAG) or gp340, is a multifunctional molecule with important functions in innate immunity, inflammation and epithelial homeostasis (PMID: 28668353). DMBT1 has some isoforms with the molecular weight of 193, 260 and 340 kDa.

实验方案

Product Specific Protocols
IF protocol for DMBT1 antibody 27069-1-APDownload protocol
IHC protocol for DMBT1 antibody 27069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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