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验证数据展示

  • WB analysis using 85946-1-RR

    WB analysis using 85946-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85946-1-RR (DLX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 85946-1-RR

    IHC staining of mouse embryo using 85946-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 85946-1-RR

    IHC staining of mouse embryo using 85946-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85946-1-RR

    Affinity and Kinetic Characterization of 85946-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85946-1-RR against Human DLX6 were performed. The affinity constant is 5.65 nM.

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DLX6 Recombinant monoclonal antibody

DLX6 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85946-1-RR

产品说明书

CloneNo. 250408B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Homeobox protein DLX-6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85946-1-RR targets DLX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19724

Product name: Recombinant human DLX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-175 aa of BC069363

Sequence: QGSNPHESDPLQGSAALSPRSPALPPVWDVSASAKGVSMPPNSYMPGYSHWYSSPHQDTMQRPQMM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 distal-less homeobox 6
别名 Homeobox protein DLX-6
计算分子量 175 aa, 20 kDa
观测分子量32 kDa
GenBank蛋白编号BC069363
基因名称 DLX6
Gene ID (NCBI) 1750
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Distal-less homeobox 6 (DLX6) has been reported to play important roles in the development of craniofacial structures, inner ear, limb, and brain. DLX6 was significantly highly expressed in oral cancer tissues in The Cancer Genome Atlas database. DLX6 promotes cell proliferation and suppresses cell apoptosis in oral cancer cells. EGFR-CCND1 pathway might be the potential mechanism participating in the regulating axis (PMID: 33215805).

实验方案

Product Specific Protocols
WB protocol for DLX6 antibody 85946-1-RRDownload protocol
IHC protocol for DLX6 antibody 85946-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols