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验证数据展示

  • WB analysis of human brain using 13046-1-AP

    WB analysis of human brain using 13046-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13046-1-AP (DLX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13046-1-AP

    IHC staining of mouse brain using 13046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13046-1-AP (DLX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13046-1-AP

    IHC staining of mouse brain using 13046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13046-1-AP (DLX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DLX1 Polyclonal antibody

DLX1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13046-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Homeobox protein DLX-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, A375 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13046-1-AP targets DLX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DLX1 fusion protein Ag3586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 distal-less homeobox 1
别名 Homeobox protein DLX-1
计算分子量 255 aa, 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC036189
基因名称 DLX1
Gene ID (NCBI) 1745
RRIDAB_2093117
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56177
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Distal-less homeobox 1(DLX1) is a member of the DLX family of homeobox transcription factors, which is essential for the production of forebrain GABAergic interneurions during embryonic development. The DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6) is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX1 possible has a role in craniofacial patterning and morphogenesis and involves in the early development of diencephalic subdivisions.

实验方案

Product Specific Protocols
WB protocol for DLX1 antibody 13046-1-APDownload protocol
IHC protocol for DLX1 antibody 13046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Manag Res

miR-489-3p Inhibits Prostate Cancer Progression by Targeting DLX1.

Authors - Peide Bai
View Article
mouseWB

Cell Death Differ

Cullin 4B-RING E3 ligase negatively regulates the immunosuppressive capacity of mesenchymal stem cells by suppressing iNOS

Authors - Ruiqi Yu
View Article