×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 31174-1-AP

    WB analysis using 31174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31174-1-AP (DLST antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31174-1-AP

    IHC staining of human placenta using 31174-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31174-1-AP (DLST antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 31174-1-AP

    IHC staining of human placenta using 31174-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31174-1-AP (DLST antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31174-1-AP

    IF Staining of HeLa using 31174-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DLST antibody (31174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DLST Polyclonal antibody

DLST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31174-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DLTS, OGDC E2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31174-1-AP targets DLST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34753

Product name: Recombinant human DLST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 212-366 aa of BC001922

Sequence: EPGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEIDMSNIQEMRARHKEAFLKKHNLKLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTITELGEKARKNELAIEDMDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
别名 DLTS, OGDC E2
计算分子量 49 kDa
观测分子量40 kDa, 49 kDa
GenBank蛋白编号BC001922
基因名称 DLST
Gene ID (NCBI) 1743
RRIDAB_3742403
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP36957
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLST (Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex) ) is the E2 transferase of α-ketoglutarate dehydrogenase complex (KGDHC) that regulates the irreversible conversion of α-ketoglutarate to succinyl-CoA in the TCA cycle.RNAi knockdown of DLST led to decreased cell viability and induction of apoptosis in human T-ALL cell lines. High DLST expression predicts poor overall and recurrencefree survival among TNBC patients (PMID: 26876595, 34785772). DLST has two isoforms of 40 kDa, 49 kDa.

实验方案

Product Specific Protocols
IF protocol for DLST antibody 31174-1-APDownload protocol
IHC protocol for DLST antibody 31174-1-APDownload protocol
WB protocol for DLST antibody 31174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...