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验证数据展示

  • WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

    WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

    IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

    IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DLL4 Polyclonal antibody, PBS Only

DLL4 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 21584-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

Delta4, hdelta2, UNQ1895/PRO4341

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

21584-1-PBS targets DLL4 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag16140

Product name: Recombinant human DLL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 317-530 aa of BC106950

Sequence: TGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLPPSFPWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 delta-like 4 (Drosophila)
别名 Delta4, hdelta2, UNQ1895/PRO4341
计算分子量 685 aa, 75 kDa
观测分子量 70-100 kDa
GenBank蛋白编号BC106950
基因名称 DLL4
Gene ID (NCBI) 54567
RRIDAB_2878888
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR61
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.

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