验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, SK-N-SH cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55056-1-AP targets DLGAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | discs, large (Drosophila) homolog-associated protein 3 |
| 别名 | DAP 3, DAP3, DAP-3, Disks large-associated protein 3, PSD 95/SAP90 binding protein 3 |
| 计算分子量 | 106 kDa |
| 观测分子量 | 106-120 kDa |
| GenBank蛋白编号 | NM_001080418 |
| 基因名称 | DLGAP3 |
| Gene ID (NCBI) | 58512 |
| RRID | AB_10858793 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95886 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DLGAP3, also named as DAP3, belongs to the SAPAP family. It may play a role in the molecular organization of synapses and neuronal cell signaling. DLGAP3 could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. It may induce enrichment of PSD-95/SAP90 at the plasma membrane. The antibody has no cross reaction to other DLGAP members.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DLGAP3 antibody 55056-1-AP | Download protocol |
| IHC protocol for DLGAP3 antibody 55056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Psychiatry Metabotropic Glutamate Receptor and Fragile X Signaling in a Female Model of Escalated Aggression. | ||
Cell Death Dis SAPAP3 regulates epileptic seizures involving GluN2A in post-synaptic densities.
| ||
Nat Neurosci Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding |
