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验证数据展示

  • WB analysis of pig brain using 67102-1-Ig

    WB analysis of pig brain using 67102-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 67102-1-Ig

    WB analysis of mouse cerebellum using 67102-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67102-1-Ig

    IHC staining of mouse brain using 67102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67102-1-Ig

    IHC staining of mouse brain using 67102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SAP102 Monoclonal antibody

SAP102 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67102-1-Ig

产品说明书

CloneNo. 1F6A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

WB, IHC, ELISA

别名

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67102-1-Ig targets SAP102 in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原 SAP102 fusion protein Ag28589 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 discs, large homolog 3 (Drosophila)
别名 Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR
计算分子量 817 aa, 90 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC093864
基因名称 SAP102
Gene ID (NCBI) 1741
RRIDAB_2882407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92796
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.

实验方案

Product Specific Protocols
WB protocol for SAP102 antibody 67102-1-IgDownload protocol
IHC protocol for SAP102 antibody 67102-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols