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验证数据展示

  • WB analysis of human testis using 20027-1-AP

    WB analysis of human testis using 20027-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20027-1-AP

    IHC staining of human testis using 20027-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DLEC1 antibody included
  • IHC staining of human testis using 20027-1-AP

    IHC staining of human testis using 20027-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with DLEC1 antibody included
  • IP experiment of PC-3 using 20027-1-AP

    IP experiment of PC-3 using 20027-1-AP

    IP result of anti-DLEC1 (IP:20027-1-AP, 4ug; Detection:20027-1-AP 1:300) with PC-3 cells lysate 1100ug.

  • IF Staining of PC-3 using 20027-1-AP

    IF Staining of PC-3 using 20027-1-AP

    Immunofluorescent analysis of PC-3 cells using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DLEC1 Polyclonal antibody

DLEC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20027-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

DLC1, F56, DLC-1, DLC 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, A549 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy DLEC1 Ready-To-Use IHC Kit. DLEC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20027-1-AP targets DLEC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deleted in lung and esophageal cancer 1
别名 DLC1, F56, DLC-1, DLC 1
计算分子量 196 kDa
观测分子量 80-90 kDa, 46 kDa
GenBank蛋白编号NM_007335
基因名称 DLEC1
Gene ID (NCBI) 9940
RRIDAB_10664924
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLEC1, also named as DLC1 F56, may act as a tumor suppressor by inhibiting cell proliferation.

实验方案

Product Specific Protocols
IF protocol for DLEC1 antibody 20027-1-APDownload protocol
IHC protocol for DLEC1 antibody 20027-1-APDownload protocol
IP protocol for DLEC1 antibody 20027-1-APDownload protocol
WB protocol for DLEC1 antibody 20027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Hum Genet

Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.

Authors - Ruijie Ming
View Article
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