验证数据展示

WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.
WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DLAT antibody included
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DLAT antibody included
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DLAT antibody included
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DLAT antibody included
IP experiment of HepG2 using 13426-1-AP
IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.
WB analysis of HAP1 using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:60000; room temperature for 1.5 hours) with wild-type and DLAT knockout HAP1 cells.
IHC staining of human colon cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DLAT antibody included
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO验证

DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13426-1-AP

产品说明书

引用文献(98)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2, PDC-E2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (98)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, A431 cells, HAP1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, LNCaP cells, MCF-7 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy DLAT Ready-To-Use IHC Kit. DLAT primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 68 publications below
IHC	See 25 publications below
IF	See 33 publications below
IP	See 2 publications below
CoIP	See 2 publications below

产品信息

13426-1-AP targets DLAT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, rabbit, monkey
免疫原	CatNo: Ag4226 Product name: Recombinant human DLAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC039084 Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	dihydrolipoamide S-acetyltransferase
别名	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2, PDC-E2
计算分子量	647 aa, 70 kDa
观测分子量	69-70 kDa
GenBank蛋白编号	BC039084
基因名称	DLAT
Gene ID (NCBI)	1737
RRID	AB_2091774
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P10515
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
IF protocol for DLAT antibody 13426-1-AP	Download protocol
IHC protocol for DLAT antibody 13426-1-AP	Download protocol
IP protocol for DLAT antibody 13426-1-AP	Download protocol
WB protocol for DLAT antibody 13426-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
mouse	WB	Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis Authors - View Article
mouse,human	WB,IF,CoIP	Mol Cell Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide Authors - Marta Russo View Article
human	WB,IHC,IF	Adv Sci (Weinh) High-Efficiency Carriers' Separation Strategy Based Ultrasmall-Bandgap CuWO4 Sono-Enhances GSH Antagonism for Cuproptosis Cascade Immunotherapy Authors - Lichao Zhu View Article
mouse	WB,IF	J Immunother Cancer C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance Authors - Hong Yang View Article
mouse	WB,IF	Adv Healthc Mater Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma Authors - Xiaotong Xu View Article

验证数据展示

WB analysis using 13426-1-AP

WB analysis of HeLa using 13426-1-AP

WB analysis using 13426-1-AP

IHC staining of human liver cancer using 13426-1-AP

IHC staining of human liver cancer using 13426-1-AP

IHC staining of mouse kidney using 13426-1-AP

IHC staining of mouse kidney using 13426-1-AP

IP experiment of HepG2 using 13426-1-AP

WB analysis of HAP1 using 13426-1-AP

IHC staining of human colon cancer using 13426-1-AP

IF Staining of HepG2 using 13426-1-AP

IF Staining of HepG2 using 13426-1-AP

DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide

High-Efficiency Carriers' Separation Strategy Based Ultrasmall-Bandgap CuWO4 Sono-Enhances GSH Antagonism for Cuproptosis Cascade Immunotherapy

C5a/C5aR pathway blocking promoted CuS-mediated cancer therapy effect by inhibiting cuproptosis resistance

Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma