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DKK4 Polyclonal antibody

DKK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27080-1-AP

Print datasheet

Synonyms

Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4

验证数据展示

  • WB analysis of mouse cerebellum using 27080-1-AP

    WB analysis of mouse cerebellum using 27080-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27080-1-AP (DKK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus using 27080-1-AP

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human oesophagus using 27080-1-AP

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse cerebellum tissue
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27080-1-AP is DKK4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen DKK4 fusion protein Ag24209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dickkopf homolog 4 (Xenopus laevis)
Synonyms Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC107047
Gene Symbol DKK4
Gene ID (NCBI) 27121
RRIDAB_2880746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DKK4 antibody 27080-1-APDownload protocol
IHC protocol for DKK4 antibody 27080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Clin Transl Med

O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signalling

Authors - Zhongqi Cui
View Article