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  • Featured Product
  • KD/KO Validated

DKC1 Polyclonal antibody

DKC1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25420-1-AP

Print datasheet

Synonyms

CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101

验证数据展示

  • WB analysis of HepG2 using 25420-1-AP

    WB analysis of HepG2 using 25420-1-AP

    WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells.

  • IF Staining of HepG2 using 25420-1-AP

    IF Staining of HepG2 using 25420-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 25420-1-AP

    IHC staining of human colon cancer using 25420-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DKC1 antibody included
  • IHC staining of human colon cancer using 25420-1-AP

    IHC staining of human colon cancer using 25420-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DKC1 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DKC1 Ready-To-Use IHC Kit. DKC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25420-1-AP is DKC1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DKC1 fusion protein Ag7992 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dyskeratosis congenita 1, dyskerin
Synonyms CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC009928
Gene Symbol DKC1
Gene ID (NCBI) 1736
RRIDAB_2880071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60832
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DKC1 antibody 25420-1-APDownload protocol
IHC protocol for DKC1 antibody 25420-1-APDownload protocol
IF protocol for DKC1 antibody 25420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cells

H/ACA Box Small Nucleolar RNA 7A promotes the Self-Renewal of Human Umbilical Cord Mesenchymal Stem Cells.

Authors - Yan Zhang
View Article
humanWB

Cell Rep

Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex.

Authors - Cai Han
  • KD Validated
View Article