验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag4904 Product name: Recombinant human DIXDC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-472 aa of BC033034 Sequence: VNPEEQLIIIQSRLDQSMEENQDLKKELLKCKQEARNLQGIKDALQQRLTQQDTSVLQLKQELLRANMDKDELHNQNVDLQRKLDERNRLLGEYKKELGQKDRLLQQHQAKLEEALRKLSDVSYHQVDLERELEHKDVLLAHCMKREADEATNYNSHNSQSNGFLLPTAGKGATSVSNRGTSDLQLVRDALRSLRNSFSGHDPQHHTIDSLEQGISSLMERLHVMETQKKQERKVRVKSPRTQVGSEYRESWPPNSKLPHSQSSPTVSSTCTKVLYFTDRSLTPFMVNIPKRLEEVTLKDFKAAIDREGNHRYHFKALDPEFGTVKEEIFHDDDAIPGWEGKIVAWVEEDHGEN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DIX domain containing 1 |
| 别名 | CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735 |
| 计算分子量 | 683 aa, 77 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC033034 |
| 基因名称 | DIXDC1 |
| Gene ID (NCBI) | 85458 |
| RRID | AB_2091188 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q155Q3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DIXDC1 antibody 13816-1-AP | Download protocol |
| WB protocol for DIXDC1 antibody 13816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Neurobiol Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency. | ||
Mol Carcinog The oncogenic role and regulatory mechanism of ACAA2 in human ovarian cancer |
