验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy DIS3L2 Ready-To-Use IHC Kit. DIS3L2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67623-1-Ig targets DIS3L2 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag29992 Product name: Recombinant human DIS3L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC030113 Sequence: AGALGYRERLDMAPDTLQKQADHCNDSRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAEYTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DIS3 mitotic control homolog (S. cerevisiae)-like 2 |
| 别名 | DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2 |
| 计算分子量 | 885 aa, 99 kDa |
| 观测分子量 | 65 kDa, 99 kda |
| GenBank蛋白编号 | BC030113 |
| 基因名称 | DIS3L2 |
| Gene ID (NCBI) | 129563 |
| RRID | AB_2882825 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8IYB7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for DIS3L2 antibody 67623-1-Ig | Download protocol |
| WB protocol for DIS3L2 antibody 67623-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential | ||
Cell Rep Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs |
