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验证数据展示

  • WB analysis using 67623-1-Ig

    WB analysis using 67623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • FC experiment of Jurkat using 67623-1-Ig

    FC experiment of Jurkat using 67623-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DIS3L2 Monoclonal antibody (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DIS3L2 Monoclonal antibody

DIS3L2 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67623-1-Ig

产品说明书

CloneNo. 2C9F2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DIS3L2 Ready-To-Use IHC Kit. DIS3L2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67623-1-Ig targets DIS3L2 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag29992

Product name: Recombinant human DIS3L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-170 aa of BC030113

Sequence: AGALGYRERLDMAPDTLQKQADHCNDSRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAEYTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DIS3 mitotic control homolog (S. cerevisiae)-like 2
别名 DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2
计算分子量 885 aa, 99 kDa
观测分子量 65 kDa, 99 kda
GenBank蛋白编号BC030113
基因名称 DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2882825
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8IYB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

实验方案

Product Specific Protocols
WB protocol for DIS3L2 antibody 67623-1-IgDownload protocol
FC protocol for DIS3L2 antibody 67623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential

Authors - Damian M Janecki
View Article