验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human placenta tissue, MCF-7 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
产品信息
12634-1-AP targets DIRAS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | DIRAS1 fusion protein Ag3329 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DIRAS family, GTP-binding RAS-like 1 |
| 别名 | Di Ras1, DIRAS1, GBTS1, GTP binding protein Di Ras1, RIG |
| 计算分子量 | 198 aa, 22 kDa |
| 观测分子量 | 22-24 kDa |
| GenBank蛋白编号 | BC030660 |
| 基因名称 | DIRAS1 |
| Gene ID (NCBI) | 148252 |
| RRID | AB_2292771 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95057 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DIRAS1 belongs to the small guanosine triphosphatase (GTPase) Ras superfamily. DIRAS1 is frequently downregulated in human cancers due to DNA copy number loss, loss of heterozygosity (LOH), and hypermethylation of the promoter. The restoration of DIRAS1 has been reported to suppress tumor growth and metastasis in human neural tumors, esophageal cancer, colorectal cancer, and ovarian cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| IHC protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| IP protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas. | ||
Mol Ther Nucleic Acids RNAa and Vector-Mediated Overexpression of DIRAS1 Suppresses Tumor Growth and Migration in Renal Cell Carcinoma.
| ||
J Biol Chem The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases. | ||
Int J Environ Res Public Health Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat. | ||
Cancer Biol Ther M6A modification regulates tumor suppressor DIRAS1 expression in cervical cancer cells |
