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验证数据展示

  • WB analysis of Raji using 10183-1-AP (same clone as 10183-1-PBS)

    WB analysis of Raji using 10183-1-AP (same clone as 10183-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10183-1-AP (DIDO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

    IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIDO1 antibody included
  • IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

    IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIDO1 antibody included
  • IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

    IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DIDO1 antibody (10183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

DIDO1 Polyclonal antibody, PBS Only

DIDO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10183-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BYE1, C20orf158, DATF 1, DATF1, DATF-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DIDO1 Ready-To-Use IHC Kit. DIDO1 primary antibody included.

产品信息

10183-1-PBS targets DIDO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0237

Product name: Recombinant human DIDO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 345-544 aa of BC000770

Sequence: ADGTDCTSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKPSLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACESSTPSWASDHNYNAVKPEKTAAPSPSLLYKCSGKYLYSLHPSLIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death inducer-obliterator 1
别名 BYE1, C20orf158, DATF 1, DATF1, DATF-1
计算分子量 244 kDa
观测分子量 300-350 kDa
GenBank蛋白编号BC000770
基因名称 DIDO1
Gene ID (NCBI) 11083
RRIDAB_2277375
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTC0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. DIDO1 has multiple isoforms with molecular weights of 243 kd, 129 kd, 59 kd, and 61 kd. DIDO1 undergoes various modifications, including phosphorylation and ubiquitination, resulting in a mainstream molecular weight of 300 kd.

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