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  • KD/KO Validated

DICER1 Polyclonal antibody

DICER1 Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC,IF-P, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20567-1-AP

Print datasheet

Synonyms

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928



经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human ovary tumor tissue
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20567-1-AP targets DICER1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC,IF-P, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dicer 1, ribonuclease type III
Synonyms DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928
Calculated Molecular Weight 219 kDa
Observed Molecular Weight 220-250 kDa, 90 kDa
GenBank Accession NumberNM_030621
Gene Symbol DICER1
Gene ID (NCBI) 23405
RRIDAB_10694287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.

实验方案

Product Specific Protocols
WB protocol for DICER1 antibody 20567-1-APDownload protocol
IHC protocol for DICER1 antibody 20567-1-APDownload protocol
IF protocol for DICER1 antibody 20567-1-APDownload protocol
IP protocol for DICER1 antibody 20567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
  • KD Validated
humanWB

Nat Chem Biol

Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation.

Authors - Jinghui Song
humanWB

Nat Commun

RNA structure probing reveals the structural basis of Dicer binding and cleavage.

Authors - Qing-Jun Luo
humanWB,IP

Nucleic Acids Res

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Authors - Xiaochuan Liu
humanWB

Nat Commun

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Authors - Chen Xu
humanWB

J Pathol

Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.

Authors - Anglesio M S MS
  • KO Validated