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验证数据展示

  • WB analysis using 88165-1-RR (same clone as 88165-1-PBS)

    WB analysis using 88165-1-RR (same clone as 88165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88165-1-RR (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88165-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 88165-1-RR (same clone as 88165-1-PBS)

    IHC staining of human skin using 88165-1-RR (same clone as 88165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 88165-1-RR (DHX9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88165-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHX9 antibody included
  • IHC staining of human skin using 88165-1-RR (same clone as 88165-1-PBS)

    IHC staining of human skin using 88165-1-RR (same clone as 88165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 88165-1-RR (DHX9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88165-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHX9 antibody included

DHX9 Recombinant monoclonal antibody, PBS Only

DHX9 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 88165-1-PBS

产品说明书

CloneNo. 260493F8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13, LKP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DHX9 Ready-To-Use IHC Kit. DHX9 primary antibody included.

产品信息

88165-1-PBS targets DHX9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12807

Product name: Recombinant human DHX9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC014246

Sequence: MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAH (Asp-Glu-Ala-His) box polypeptide 9
别名 ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13, LKP
计算分子量 1270 aa, 141 kDa
观测分子量 140 kDa
GenBank蛋白编号BC014246
基因名称 DHX9
Gene ID (NCBI) 1660
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08211
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain (PMID:20569003). It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2 (PMID: 19029303, 22190748).

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