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验证数据展示

  • WB analysis of Jurkat using 11021-1-AP

    WB analysis of Jurkat using 11021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11021-1-AP

    IHC staining of human lymphoma using 11021-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11021-1-AP (DHX16 antibody) at dilution of 1:10 (under 10x lens).

  • IF Staining of MCF-7 using 11021-1-AP

    IF Staining of MCF-7 using 11021-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DHX16 antibody 11021-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

DHX16 Polyclonal antibody

DHX16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11021-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

EC:3.6.4.13, DEAH-box protein 16, DEAH box protein 16, DDX16, DBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, human brain tissue, mouse testis tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11021-1-AP targets DHX16 in WB, IHC, IF/ICC, Neutralization, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用Neutralization
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag1482

Product name: Recombinant human DHX16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC009392

Sequence: MATPAGLERWVQDELHSVLGLSERHVAQFLIGTAQRCTSAEEFVQRLRDTDTLDLSGPARDFALRLWNKVPRKAVVEKPARAAEREARALLEKNRSYRLLEDSEESSEETVSRAGSSLQKKRKKRKHLRKKREEEEEEEASEKGKKKTGGSKQQTEKPESEDEWERTERERLQDLEERDAFAERVRQRDKDRTRNVLERSDKKAYEEAQKRLKMAEEDRKAMVPELRKKSRREYLAKREREKLEDLEAELADEEFLFGDVELSRHERQELKYKRRVRDLAREYRAAGEQEKLEATNRYHMPKETRGQPAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAH (Asp-Glu-Ala-His) box polypeptide 16
别名 EC:3.6.4.13, DEAH-box protein 16, DEAH box protein 16, DDX16, DBP2
计算分子量 119 kDa
观测分子量 119 kDa
GenBank蛋白编号BC009392
基因名称 DHX16
Gene ID (NCBI) 8449
RRIDAB_2245938
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60231
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DHX16 antibody 11021-1-APDownload protocol
IHC protocol for DHX16 antibody 11021-1-APDownload protocol
IF protocol for DHX16 antibody 11021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanNeutralization

Nat Commun

Molecular basis for the activation of human spliceosome

Authors - Xiechao Zhan
View Article