DHX15 Recombinant antibody
DHX15 Recombinant Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F13
验证数据展示
经过测试的应用
Positive WB detected in | U-251 cells, HeLa cells, U2OS cells, A431 cells, NIH/3T3 cells, C6 cells |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:1000-1:4000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82137-1-RR targets DHX15 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | DHX15 fusion protein Ag2895 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 15 |
Synonyms | ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p |
Calculated Molecular Weight | 795 aa, 91 kDa |
Observed Molecular Weight | 90-95 kDa |
GenBank Accession Number | BC035974 |
Gene Symbol | DHX15 |
Gene ID (NCBI) | 1665 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O43143 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DHX15 antibody 82137-1-RR | Download protocol |
IHC protocol for DHX15 antibody 82137-1-RR | Download protocol |
IF protocol for DHX15 antibody 82137-1-RR | Download protocol |
IP protocol for DHX15 antibody 82137-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |