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验证数据展示

  • WB analysis using 17279-1-AP

    WB analysis using 17279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17279-1-AP (DHRSX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17279-1-AP

    IP experiment of HeLa using 17279-1-AP

    IP result of anti-DHRSX (IP:17279-1-AP, 4ug; Detection:17279-1-AP 1:400) with HeLa cells lysate 1320 ug.

DHRSX Polyclonal antibody

DHRSX Polyclonal Antibody for WB, IP, ELISA
Cat No. 17279-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CXorf11, DHRS5X, DHRS5Y, DHRSX, DHRSXY, DHRSY, SDR46C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17279-1-AP targets DHRSX in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11046

Product name: Recombinant human DHRSX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC032340

Sequence: MSPLSAARAALRVYAVGAAVILAQLLRRCRGGFLEPVFPPRPDRVAIVTGGTDGIGYSTAKHLARLGMHVIIAGNNDSKAKQVVSKIKEETLNDKVEFLYCDLASMTSIRQFVQKFKMKKIPLHVLINNAGVMMVPQRKTRDGFEEHFGLNYLGHFLLTNLLLDTLKESGSPGHSARVVTVSSATHYVAELNMDDLQSSACYSPHAAYAQSKLALVLFTYHLQRLLAAEGSHVTANVVDPGVVNTDLYKHVFWATRLAKKLLGWLLFKTPDEGAWTSIYAAVTPELEGVGGRYLYNEKETKSLHVTYNQKLQQQLWSKSCEMTGVLDVTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) X-linked
别名 CXorf11, DHRS5X, DHRS5Y, DHRSX, DHRSXY, DHRSY, SDR46C1
计算分子量 330 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC032340
基因名称 DHRSX
Gene ID (NCBI) 207063
RRIDAB_3085520
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5I4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRSX(Dehydrogenase/reductase SDR family member on chromosome X) is also named as CXorf11, DHRS5X and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.

实验方案

Product Specific Protocols
WB protocol for DHRSX antibody 17279-1-APDownload protocol
IP protocol for DHRSX antibody 17279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols