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验证数据展示

  • WB analysis using 15530-1-AP

    WB analysis using 15530-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15530-1-AP (DHRS7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 15530-1-AP

    IHC staining of mouse colon using 15530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 15530-1-AP

    IHC staining of mouse colon using 15530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHRS7B Polyclonal antibody

DHRS7B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15530-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells, PC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15530-1-AP targets DHRS7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DHRS7B fusion protein Ag7690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 7B
别名 CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1
计算分子量 35 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC004126
基因名称 DHRS7B
Gene ID (NCBI) 25979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IAN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS7B (Dehydrogenase/Reductase 7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions, particularly the metabolism of steroids, lipids, and xenobiotics. DHRS7B is characterized by its NAD(P)H-dependent oxidoreductase activity and plays roles in cellular detoxification, lipid homeostasis, and possibly steroid hormone regulation.

实验方案

Product Specific Protocols
WB protocol for DHRS7B antibody 15530-1-APDownload protocol
IHC protocol for DHRS7B antibody 15530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols