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验证数据展示

  • WB analysis using 15530-1-AP

    WB analysis using 15530-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15530-1-AP (DHRS7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 15530-1-AP

    IF Staining of U2OS using 15530-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DHRS7B antibody (15530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse colon using 15530-1-AP

    IHC staining of mouse colon using 15530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 15530-1-AP

    IHC staining of mouse colon using 15530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHRS7B Polyclonal antibody

DHRS7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15530-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells, PC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15530-1-AP targets DHRS7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DHRS7B fusion protein Ag7690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 7B
别名 CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1
计算分子量 35 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC004126
基因名称 DHRS7B
Gene ID (NCBI) 25979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IAN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS7B (Dehydrogenase/Reductase 7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions, particularly the metabolism of steroids, lipids, and xenobiotics. DHRS7B is characterized by its NAD(P)H-dependent oxidoreductase activity and plays roles in cellular detoxification, lipid homeostasis, and possibly steroid hormone regulation.

实验方案

Product Specific Protocols
WB protocol for DHRS7B antibody 15530-1-APDownload protocol
IHC protocol for DHRS7B antibody 15530-1-APDownload protocol
IF protocol for DHRS7B antibody 15530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols