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验证数据展示

  • WB analysis using 15061-1-AP

    WB analysis using 15061-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 15061-1-AP (DHRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skeletal muscle using 15061-1-AP

    IHC staining of rat skeletal muscle using 15061-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 15061-1-AP

    IHC staining of mouse skeletal muscle using 15061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15061-1-AP

    IF Staining of HepG2 using 15061-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS7 antibody (15061-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

DHRS7 Polyclonal antibody

DHRS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15061-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CGI 86, CGI-86, DHRS7A, EC:1.1.1.-, Protein SDR34C1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15061-1-AP targets DHRS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7064

Product name: Recombinant human DHRS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-183 aa of BC000637

Sequence: RADGDLTLLWAEWQGRRPEWELTDMVVWVTGASSGIGEELAYQLSKLGVSLVLSARRVHELERVKRRCLENGNLKEKDILVLPLDLTDTGSHEAATKAVLQEFGRIDILVNNGGMSQRSLCMDTSLDVYRKLIELNYLGTVSLTKCVLPHMIERKQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 7
别名 CGI 86, CGI-86, DHRS7A, EC:1.1.1.-, Protein SDR34C1
计算分子量 38 kDa
观测分子量 32-38 kDa
GenBank蛋白编号BC000637
基因名称 DHRS7
Gene ID (NCBI) 51635
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS7 (Dehydrogenase/Reductase (SDR family) member 7), also known as SDR34C1, retSDR4, is a member of a large family of short-chain dehydrogenases/reductases (SDR). The DHRS7 gene codes for two subtypes and is found on chromosome 14. Isotype 1 (38 kDa) has 339 amino acids, while isotype 2 (32 kDa) contains 289 amino acids (PMID: 26466768). DHRS7 has been demonstrated to be an integral protein facing the lumen of the endoplasmic reticulum with lack of posttranscriptional glycosylation modification(PMID: 24246760).

实验方案

Product Specific Protocols
IF protocol for DHRS7 antibody 15061-1-APDownload protocol
IHC protocol for DHRS7 antibody 15061-1-APDownload protocol
WB protocol for DHRS7 antibody 15061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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