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验证数据展示

  • WB analysis of rat liver using 15279-1-AP (same clone as 15279-1-PBS)

    WB analysis of rat liver using 15279-1-AP (same clone as 15279-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • WB analysis using 15279-1-AP (same clone as 15279-1-PBS)

    WB analysis using 15279-1-AP (same clone as 15279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

    IP experiment of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

    IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

DHRS4 Polyclonal antibody, PBS Only

DHRS4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15279-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15279-1-PBS targets DHRS4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7398

Product name: Recombinant human DHRS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-278 aa of BC003019

Sequence: MHKAGLLGLCARAWNSVRMASSGMTRRDPLANKVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGKAEDRERLVATAVKLHGGIDILVSNAAVNPFFGSIMDVTEEVWDKTLDINVKAPALMTKAVVPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCLAPGLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDASYITGETVVVGGGTPSRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 4
别名
计算分子量 30 kDa
观测分子量 29 kDa
GenBank蛋白编号BC003019
基因名称 DHRS4
Gene ID (NCBI) 10901
RRIDAB_2292856
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTZ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).

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