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验证数据展示

  • IHC staining of human kidney using 16275-1-AP

    IHC staining of human kidney using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 16275-1-AP

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16275-1-AP

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 16275-1-AP

    IF Staining of A431 using 16275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DHRS1 antibody (16275-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

DHRS1 Polyclonal antibody

DHRS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16275-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Short chain dehydrogenase/reductase family 19C member 1, SDR19C1, Protein SDR19C1, EC:1.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, human liver tissue, mouse colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16275-1-AP targets DHRS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9351

Product name: Recombinant human DHRS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC014057

Sequence: MAAPMNGQVCVVTGASRGIGRGIALQLCKAGATVYITGRHLDTLRVVAQEAQSLGGQCVPVVCDSSQESEVRSLFEQVDREQQGRLDVLVNNAYAGVQTILNTRNKAFWETPASMWDDINNVGLRGHYFCSVYGARLMVPAGQGLIVVISSPGSLQYMFNVLYGVGKAACDKLAADCAHELRRHGVSCVSLWPGIVQTELLKEHMAKEEVLQDPVLKQFKSAFSSAETTELSGKCVVALATDPNILSLSGKVLPSCDLARRYGLRDVDGRPVQDYLSLSSVLSHVSGLGWLASYLPSFLRVPKWIIALYTSKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 1
别名 Short chain dehydrogenase/reductase family 19C member 1, SDR19C1, Protein SDR19C1, EC:1.1.1.-
计算分子量 313 aa, 34 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC014057
基因名称 DHRS1
Gene ID (NCBI) 115817
RRIDAB_2292847
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS1(Dehydrogenase/reductase SDR family member 1) is also named as LJ25430, MGC20204, DKFZp586I0523, SDR19C1, FLJ14250 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.Northern blot analysis detected DHRS1express in heart highest and in liver lowest.

实验方案

Product Specific Protocols
WB protocol for DHRS1 antibody 16275-1-APDownload protocol
IHC protocol for DHRS1 antibody 16275-1-APDownload protocol
IF protocol for DHRS1 antibody 16275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols