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验证数据展示

  • WB analysis using 15099-1-AP

    WB analysis using 15099-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15099-1-AP (DHDDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15099-1-AP

    IHC staining of mouse testis using 15099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15099-1-AP

    IHC staining of mouse testis using 15099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 15099-1-AP

    IHC staining of rat testis using 15099-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHDDS Polyclonal antibody

DHDDS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15099-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Cis-IPTase, Cis-isoprenyltransferase, Cis-prenyltransferase subunit hCIT, Dehydrodolichyl diphosphate synthase complex subunit DHDDS, EC:2.5.1.87

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15099-1-AP targets DHDDS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DHDDS fusion protein Ag7426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrodolichyl diphosphate synthase
别名 Cis-IPTase, Cis-isoprenyltransferase, Cis-prenyltransferase subunit hCIT, Dehydrodolichyl diphosphate synthase complex subunit DHDDS, EC:2.5.1.87
计算分子量 39 kDa
观测分子量 34 kDa
GenBank蛋白编号BC003643
基因名称 DHDDS
Gene ID (NCBI) 79947
RRIDAB_2091576
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86SQ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHDDS (dehydrodolichol diphosphate synthetase) encodes a protein involved in dolichol biosynthesis on the cytoplasmic face of the ER, where N-glycosylation, O-mannosylation, C-mannosylation and GPI-anchor synthesis occurs (PMID: 34382076). Homozygous mutations in the DHDDS gene (c.124 A>G) have been associated with nonsyndromic retinitis pigmentosa in consanguineous families (PMID: 36628425).

实验方案

Product Specific Protocols
WB protocol for DHDDS antibody 15099-1-APDownload protocol
IHC protocol for DHDDS antibody 15099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols