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验证数据展示

  • IHC staining of human skin cancer using 16459-1-AP

    IHC staining of human skin cancer using 16459-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16459-1-AP (dGK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 16459-1-AP

    IP experiment of mouse kidney using 16459-1-AP

    IP result of anti-dGK (IP:16459-1-AP, 3ug; Detection:16459-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

dGK Polyclonal antibody

dGK Polyclonal Antibody for IHC, IP, ELISA
Cat No. 16459-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IP, ELISA

别名

DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16459-1-AP targets dGK in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9501

Product name: Recombinant human DGUOK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-277 aa of BC015757

Sequence: MAAGRLFLSRLRAPFSSMAKSPLEGVSSSRGLHAGRGPRRLSIEGNIAVGKSTFVKLLTKTYPEWHVATEPVATWQNIQAAGTQKACTAQSLGNLLDMMYREPARWSYTFQTFSFLSRLKVQLEPFPEKLLQARKPVQIFERSVYSDRYIFAKNLFENGSLSDIEWHIYQDWHSFLLWEFASRITLHGFIYLQASPQVCLKRLYQRAREEEKGIELAYLEQLHGQHEAWLIHKTTKLHFEALMNIPVLVLDVNDDFSEEVTKQEDLMREVNTFVKNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyguanosine kinase
别名 DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial
计算分子量 277 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC015757
基因名称 DGUOK
Gene ID (NCBI) 1716
RRIDAB_2878260
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for dGK antibody 16459-1-APDownload protocol
IP protocol for dGK antibody 16459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols