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dGK Polyclonal antibody

dGK Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16314-1-AP

Print datasheet

Synonyms

Deoxyguanosine kinase, dGK, DGUOK

验证数据展示

  • WB analysis of mouse kidney using 16314-1-AP

    WB analysis of mouse kidney using 16314-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 16314-1-AP

    IHC staining of human hepatocirrhosis using 16314-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16314-1-AP targets dGK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen dGK fusion protein Ag9361 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyguanosine kinase
Synonyms Deoxyguanosine kinase, dGK, DGUOK
Calculated Molecular Weight 277 aa, 32 kDa
Observed Molecular Weight 28-32 kDa, 56-64 kDa
GenBank Accession NumberBC015757
Gene Symbol DGUOK
Gene ID (NCBI) 1716
RRIDAB_10638311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)

实验方案

Product Specific Protocols
WB protocol for dGK antibody 16314-1-APDownload protocol
IHC protocol for dGK antibody 16314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols