• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84830-5-RR (same clone as 84830-5-PBS)

    WB analysis using 84830-5-RR (same clone as 84830-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84830-5-RR (DGKZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84830-5-RR (same clone as 84830-5-PBS)

    IF Staining of A431 using 84830-5-RR (same clone as 84830-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (84830-5-RR, Clone: 241444A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

DGKZ Recombinant antibody, PBS Only

DGKZ Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84830-5-PBS

产品说明书

CloneNo. 241444A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

241444A2, DAG kinase zeta, DAGK5, DAGK6, DGK ZETA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84830-5-PBS targets DGKZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DGKZ fusion protein Ag37336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 diacylglycerol kinase, zeta 104kDa
别名 241444A2, DAG kinase zeta, DAGK5, DAGK6, DGK ZETA
计算分子量104kDa，928aa
观测分子量 104-124 kDa
GenBank蛋白编号NM_001199267.1
基因名称 DGKZ
Gene ID (NCBI) 8525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13574
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).