• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DGKQ Polyclonal antibody

DGKQ Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17885-1-AP

Print datasheet

Synonyms

DAG kinase theta, DAGK, DAGK4, DAGK7, DGK theta, DGKQ, Diacylglycerol kinase theta, Diglyceride kinase theta

验证数据展示

  • WB analysis of mouse brain using 17885-1-AP

    WB analysis of mouse brain using 17885-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 17885-1-AP

    WB analysis using 17885-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17885-1-AP

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DGKQ antibody included
  • IHC staining of human colon cancer using 17885-1-AP

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DGKQ antibody included

经过测试的应用

Positive WB detected inmouse brain tissue, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human cervix tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DGKQ Ready-To-Use IHC Kit. DGKQ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17885-1-AP targets DGKQ in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DGKQ fusion protein Ag11938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diacylglycerol kinase, theta 110kDa
Synonyms DAG kinase theta, DAGK, DAGK4, DAGK7, DGK theta, DGKQ, Diacylglycerol kinase theta, Diglyceride kinase theta
Calculated Molecular Weight 942 aa, 101 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC063801
Gene Symbol DGKQ
Gene ID (NCBI) 1609
RRIDAB_2091305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52824
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DGKQ antibody 17885-1-APDownload protocol
IHC protocol for DGKQ antibody 17885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
  • KO Validated
View Article
WB

J Sci Food Agric

Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway

Authors - Guangyi Bai
View Article