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验证数据展示

  • WB analysis of human testis using 20090-1-AP

    WB analysis of human testis using 20090-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20090-1-AP

    IF Staining of HepG2 using 20090-1-AP

    Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

DGKK-Specific Polyclonal antibody

DGKK-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20090-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DGKK, EC:2.7.1.107, DGK-kappa, DGK kappa, DAG kinase kappa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, HuH-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20090-1-AP targets DGKK-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diacylglycerol kinase, kappa
别名 DGKK, EC:2.7.1.107, DGK-kappa, DGK kappa, DAG kinase kappa
计算分子量 142 kDa
观测分子量 135-140 kDa
GenBank蛋白编号NM_001013742
基因名称 DGKK
Gene ID (NCBI) 139189
RRIDAB_10666998
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5KSL6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.

实验方案

Product Specific Protocols
WB protocol for DGKK-Specific antibody 20090-1-APDownload protocol
IF protocol for DGKK-Specific antibody 20090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols