验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, HuH-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20090-1-AP targets DGKK-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diacylglycerol kinase, kappa |
| 别名 | DGKK, EC:2.7.1.107, DGK-kappa, DGK kappa, DAG kinase kappa |
| 计算分子量 | 142 kDa |
| 观测分子量 | 135-140 kDa |
| GenBank蛋白编号 | NM_001013742 |
| 基因名称 | DGKK |
| Gene ID (NCBI) | 139189 |
| RRID | AB_10666998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5KSL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DGKK-Specific antibody 20090-1-AP | Download protocol |
| WB protocol for DGKK-Specific antibody 20090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
