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验证数据展示

  • WB analysis using 11900-1-AP (same clone as 11900-1-PBS)

    WB analysis using 11900-1-AP (same clone as 11900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11900-1-AP (DGKE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 11900-1-AP (same clone as 11900-1-PBS)

    IF Staining of A431 using 11900-1-AP (same clone as 11900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKE antibody (11900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

DGKE Polyclonal antibody, PBS Only

DGKE Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11900-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11900-1-PBS targets DGKE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2512

Product name: Recombinant human DGKE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-177 aa of BC022297

Sequence: MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRYGLRGHSLSQNAPWESGFHRVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diacylglycerol kinase, epsilon 64kDa
别名
计算分子量 64 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC022297
基因名称 DGKE
Gene ID (NCBI) 8526
RRIDAB_2091145
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52429
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.

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