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  • KD/KO Validated

DGCR8 C-terminal Polyclonal antibody

DGCR8 C-terminal Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)


FC, IF/ICC, IHC, IP, WB, ELISA and More (3)



Cat No : 10996-1-AP

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C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1


Positive WB detected inHEK-293 cells, A431 cells, mouse testis tissue, Jurkat cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells, SH-SY5Y cells


Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


10996-1-AP targets DGCR8 C-terminal in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, RIP, IHC, CoIP, ChIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen DGCR8 C-terminal fusion protein Ag1429 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DiGeorge syndrome critical region gene 8
Synonyms C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1
Calculated Molecular Weight 773 aa, 86 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009323
Gene Symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).


Product Specific Protocols
WB protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IHC protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IF protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IP protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
Standard Protocols
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