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  • KD/KO Validated

DGCR8 C-terminal Polyclonal antibody

DGCR8 C-terminal Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10996-1-AP

Print datasheet

Synonyms

C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1



经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, mouse testis tissue, Jurkat cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells, SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10996-1-AP targets DGCR8 C-terminal in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, RIP, IHC, CoIP, ChIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen DGCR8 C-terminal fusion protein Ag1429 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DiGeorge syndrome critical region gene 8
Synonyms C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1
Calculated Molecular Weight 773 aa, 86 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009323
Gene Symbol DGCR8
Gene ID (NCBI) 54487
RRIDAB_2090987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

实验方案

Product Specific Protocols
WB protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IHC protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IF protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IP protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Nature

Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated
humanWB

Nature

Modulation of microRNA processing by p53.

Authors - Suzuki Hiroshi I HI
humanWB

Cell

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.

Authors - Piskounova Elena E
humanWB

Cell

Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer.

Authors - Masaki Mori
mouseChIP

Cell

Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis.

Authors - Hiroshi I Suzuki
humanWB

Cell Stem Cell

Principles of signaling pathway modulation for enhancing human naive pluripotency induction.

Authors - Jonathan Bayerl