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验证数据展示

  • WB analysis using 17100-1-AP (same clone as 17100-1-PBS)

    WB analysis using 17100-1-AP (same clone as 17100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17100-1-AP (DGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 17100-1-AP (same clone as 17100-1-PBS)

    IHC staining of human liver using 17100-1-AP (same clone as 17100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17100-1-AP (DGAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17100-1-PBS in a different storage buffer formulation.

  • WB analysis using 17100-1-AP (same clone as 17100-1-PBS)

    WB analysis using 17100-1-AP (same clone as 17100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17100-1-AP (DGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17100-1-PBS in a different storage buffer formulation.

DGAT2 Polyclonal antibody, PBS Only

DGAT2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17100-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:2.3.1.76, EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17100-1-PBS targets DGAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10719

Product name: Recombinant human DGAT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-388 aa of BC015234

Sequence: DWNTPKKGGRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVSRDTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLRNRKGFVKLALRHGADLVPIYSFGENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITIPKLEHPTQQDIDLYHTMYMEALVKLFDKHKTKFGLPETEVLEVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diacylglycerol O-acyltransferase homolog 2 (mouse)
别名 EC:2.3.1.76, EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT
计算分子量 388 aa, 44 kDa
观测分子量39~44kDa
GenBank蛋白编号BC015234
基因名称 DGAT2
Gene ID (NCBI) 84649
RRIDAB_2918049
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PD7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Diacylglycerol acyltransferase 2 (DGAT2) is an integral membrane protein and major enzyme that catalyzes the final step of triacylglycerol (TG) synthesis in eukaryotes (PMID: 21680734). DGAT2 is present in mitochondria-associated membranes, specialized domains of the ER that are highly enriched in lipid synthetic enzymes (PMID: 19049983). DGAT2 isoforms (39 kDa, 44kDa) have major differences in acyl-CoA substrate specificity toward erucoyl-CoA.

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