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验证数据展示

  • WB analysis using 30696-1-AP

    WB analysis using 30696-1-AP

    Non-treated and actinomycin D treated Neuro-2a cells were subjected to SDS PAGE followed by western blot with 30696-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30696-1-AP

    WB analysis using 30696-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30696-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DFNA5/GSDME Polyclonal antibody

DFNA5/GSDME Polyclonal Antibody for WB, ELISA
Cat No. 30696-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

Cleaved DFNA5, Cleaved DFNA5/GSDME, Cleaved Gasdermin E, Cleaved GSDME, Dfna5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells, J774A.1 cells, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30696-1-AP targets DFNA5/GSDME in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag33622

Product name: Recombinant mouse Dfna5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC132303

Sequence: MFAKATRNFLKEVDAGGDLISVSHLNDSDKLQLLSLVTKKKRYWCWQRPKYQILSATLEDVLTEGHCLSPVVVESDFVKYESKCENHKSGAIGTVVGKVKLNVGGKGVVESHSSFGTLRKQEVDVQQLIQDAVKRTVNMDNLVLQQVLESRNEVLCVLTQKIMTTQKCVISEHVQSEETCGGMVGIQTKTIQVSATEDGT*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5 (human)
别名 Cleaved DFNA5, Cleaved DFNA5/GSDME, Cleaved Gasdermin E, Cleaved GSDME, Dfna5
计算分子量57 kDa
观测分子量57 kDa, 35 kDa
GenBank蛋白编号BC132303
基因名称 DFNA5/GSDME
Gene ID (NCBI) 54722
RRIDAB_3669751
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Z2D3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. 30696-1-AP can detect full-length GSDME and cleaved GSDME.

实验方案

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 30696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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