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验证数据展示

  • WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

    WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68177-1-Ig (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DFFA,DFF45 antibody (68177-1-Ig, Clone: 1F6H9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug DFFA,DFF45 Monoclonal antibody (68177-1-Ig, Clone:1F6H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50354-1

    Cytometric bead array standard curve of MP50354-1

    Cytometric bead array standard curve of MP50354-1, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-1-PBS. Detection antibody: 68177-2-PBS. Standard:Ag32657. Range: 3.125-200 ng/mL.

  • Cytometric bead array standard curve of MP50354-2

    Cytometric bead array standard curve of MP50354-2

    Cytometric bead array standard curve of MP50354-2, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-3-PBS. Detection antibody: 68177-1-PBS. Standard:Ag32657. Range: 6.25-200 ng/mL.

DFFA/DFF45 Monoclonal antibody, PBS Only (Capture/Detector)

DFFA/DFF45 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 68177-1-PBS

产品说明书

CloneNo. 1F6H9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

DFFA,DFF45, DFF45, DFFA, 1F6H9, DFF alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68177-1-PBS targets DFFA/DFF45 as part of a matched antibody pair:

MP50354-1: 68177-1-PBS capture and 68177-2-PBS detection (validated in Cytometric bead array)

MP50354-2: 68177-3-PBS capture and 68177-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32657

Product name: Recombinant human DFFA,DFF45 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 9-328 aa of BC000037

Sequence: GVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DNA fragmentation factor, 45kDa, alpha polypeptide
别名 DFFA,DFF45, DFF45, DFFA, 1F6H9, DFF alpha
计算分子量 37 kDa
观测分子量42 kDa
GenBank蛋白编号BC000037
基因名称 DFFA
Gene ID (NCBI) 1676
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO00273
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.

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