验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
25833-1-AP targets DEPP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | DEPP fusion protein Ag22773 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 10 open reading frame 10 |
| 别名 | C10orf10, DEPP, DEPP1, Fasting induced gene protein, FIG, Protein DEPP |
| 计算分子量 | 212 aa, 23 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC011402 |
| 基因名称 | DEPP |
| Gene ID (NCBI) | 11067 |
| RRID | AB_2880260 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NTK1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEPP, also named as FIG and C10orf10, is known to be differentially regulated in mouse tissues in response to hypoxia and oxidative stress. Moreover, Studies have reported that DEPP1 is one of the early adipogenic markers (PMID:36918557).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DEPP antibody 25833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma. | ||
Cell Death Dis Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.
| ||
Int J Oncol Baicalein induces the apoptosis of HCT116 human colon cancer cells via the upregulation of DEPP/Gadd45a and activation of MAPKs. | ||