验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, COLO 320 cells, HT-1080 cells |
Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21312-1-AP targets DEPDC6 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | DEPDC6 fusion protein Ag15959 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | DEP domain containing 6 |
别名 | DEP domain containing 6, DEP.6, DEPDC6, Deptor |
计算分子量 | 409 aa, 46 kDa |
观测分子量 | 55 kDa |
GenBank蛋白编号 | BC024746 |
基因名称 | DEPDC6 |
Gene ID (NCBI) | 64798 |
RRID | AB_2878837 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TB45 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.
实验方案
Product Specific Protocols | |
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WB protocol for DEPDC6 antibody 21312-1-AP | Download protocol |
IHC protocol for DEPDC6 antibody 21312-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |