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验证数据展示

  • WB analysis using 26659-1-AP

    WB analysis using 26659-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26659-1-AP (DENND4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DENND4A Polyclonal antibody

DENND4A Polyclonal Antibody for WB, ELISA
Cat No. 26659-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MYCPBP, KIAA0476, IRLB, DENN/MADD domain containing 4A, C myc promoter binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26659-1-AP targets DENND4A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 DENND4A fusion protein Ag24568 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DENN/MADD domain containing 4A
别名 MYCPBP, KIAA0476, IRLB, DENN/MADD domain containing 4A, C myc promoter binding protein
观测分子量 230 kDa
GenBank蛋白编号BC041706
基因名称 DENND4A
Gene ID (NCBI) 10260
RRIDAB_3669559
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z401
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DENND4A (DENN domain-containing protein 4A), also called IRLB and MYCPBP, is a probable guanine nucleotide exchange factor (GEF) that may activate RAB10. It can promote the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. It may bind to the ISRE-like element (interferon-stimulated response element) of the MYC P2 promoter (PMID: 20937701; 8056341).

实验方案

Product Specific Protocols
WB protocol for DENND4A antibody 26659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols