验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26659-1-AP targets DENND4A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | DENND4A fusion protein Ag24568 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DENN/MADD domain containing 4A |
| 别名 | MYCPBP, KIAA0476, IRLB, DENN/MADD domain containing 4A, C myc promoter binding protein |
| 观测分子量 | 230 kDa |
| GenBank蛋白编号 | BC041706 |
| 基因名称 | DENND4A |
| Gene ID (NCBI) | 10260 |
| RRID | AB_3669559 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z401 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DENND4A (DENN domain-containing protein 4A), also called IRLB and MYCPBP, is a probable guanine nucleotide exchange factor (GEF) that may activate RAB10. It can promote the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. It may bind to the ISRE-like element (interferon-stimulated response element) of the MYC P2 promoter (PMID: 20937701; 8056341).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DENND4A antibody 26659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |