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验证数据展示

  • WB analysis of SKOV-3 using 25658-1-AP

    WB analysis of SKOV-3 using 25658-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 25658-1-AP

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 25658-1-AP

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 25658-1-AP

    IP experiment of K-562 using 25658-1-AP

    IP result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug.

  • WB analysis using 25658-1-AP

    WB analysis using 25658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 25658-1-AP

    IF Staining of SKOV-3 using 25658-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DENND1A Polyclonal antibody

DENND1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25658-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells, A2780 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman endometrial cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25658-1-AP targets DENND1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22547

Product name: Recombinant human DENND1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 451-559 aa of BC039703

Sequence: QKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DENN/MADD domain containing 1A
别名 Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3
计算分子量 1009 aa, 111 kDa
观测分子量 111 kDa, 64 kDa
GenBank蛋白编号BC039703
基因名称 DENND1A
Gene ID (NCBI) 57706
RRIDAB_2880180
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEH3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.

实验方案

Product Specific Protocols
WB protocol for DENND1A antibody 25658-1-APDownload protocol
IHC protocol for DENND1A antibody 25658-1-APDownload protocol
IF protocol for DENND1A antibody 25658-1-APDownload protocol
IP protocol for DENND1A antibody 25658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols