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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 25991-1-AP

    WB analysis using 25991-1-AP

    NIH/3T3 cells lysates were subjected to SDS PAGE followed by western blot with 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25991-1-AP

    IF Staining of A431 using 25991-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DEM1/EXO5 antibody (25991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DEM1/EXO5 Polyclonal antibody

DEM1/EXO5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25991-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C1orf176, DEM1, EXO5, Exonuclease V

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25991-1-AP targets DEM1/EXO5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DEM1/EXO5 fusion protein Ag22666 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 defects in morphology 1 homolog (S. cerevisiae)
别名 C1orf176, DEM1, EXO5, Exonuclease V
观测分子量42 kDa
GenBank蛋白编号BC021969
基因名称 DEM1
Gene ID (NCBI) 64789
RRIDAB_3085832
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IHC protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IF protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols