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DEK Recombinant antibody

DEK Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

230065H2

Cat No : 82880-1-RR

Print datasheet

Synonyms

D6S231E, DEK, DEK oncogene, Protein DEK

验证数据展示

  • WB analysis using 82880-1-RR

    WB analysis using 82880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 82880-1-RR

    IF Staining of U2OS using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • IF Staining of HeLa using 82880-1-RR

    IF Staining of HeLa using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IF detected inU2OS cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82880-1-RR targets DEK in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, rat
Immunogen DEK fusion protein Ag8225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEK oncogene
Synonyms D6S231E, DEK, DEK oncogene, Protein DEK
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35659
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

实验方案

Product Specific Protocols
WB protocol for DEK antibody 82880-1-RRDownload protocol
IF protocol for DEK antibody 82880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols