验证数据展示
经过测试的应用
| Positive IP detected in | LNCaP cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33219-1-AP targets DEFB132 in IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37893 Product name: Recombinant human DEFB132 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-95 aa of BC137293 Sequence: GGSKCVSNTPGYCRTCCHWGETALFMCNASRKCCISYSFLPKPDLPQLIGNHWQSRRRNTQRKDKKQQTTVTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | defensin, beta 132 |
| 别名 | BD-32, Beta-defensin 132, Beta-defensin 32, DEFB32, DEFB-32 |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC137293 |
| 基因名称 | DEFB132 |
| Gene ID (NCBI) | 400830 |
| RRID | AB_3742901 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q7Z7B7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human DEFB132 (Defensin Beta 132) is a member of the beta-defensin family and functions as a secreted antimicrobial peptide. This protein is highly expressed in epididymal epithelial cells and sperm, regulated by the androgen receptor signaling pathway, and serves as a key effector molecule of innate immunity in the male reproductive tract. DEFB132 exhibits broad-spectrum antimicrobial activity, inhibiting both Gram-negative bacteria such as *Escherichia coli* and Gram-positive bacteria such as *Staphylococcus aureus*, exerting its bactericidal function by disrupting the cell membranes of pathogens. As an inducible immune effector molecule, it plays multiple biological roles in host defense, reproductive health, and metabolic homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DEFB132 antibody 33219-1-AP | Download protocol |
| IP protocol for DEFB132 antibody 33219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
