验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33236-1-AP targets DEFB119 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36816 Product name: Recombinant human DEFB119 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-84 aa of BC062212 Sequence: KRHILRCMGNSGICRASCKKNEQPYLYCRNCQSCCLQSYMRISISGKEENTDWSYEKQWPRLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | defensin, beta 119 |
| 别名 | Beta-defensin 119, Beta-defensin 120, Beta-defensin 19, Beta-defensin 20, DEFB120 |
| 计算分子量 | 84 aa, 10 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC062212 |
| 基因名称 | DEFB119 |
| Gene ID (NCBI) | 245932 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N690 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEFB119 is a reproductive tract-specific β-defensin highly expressed in the testis and the epididymis (16). Single-cell sequencing analysis of testicular cells isolated from nonobstructive azoospermia men demonstrates an elevated expression of DEFB119 in the Sertoli cells. Male mice lacking the mouse ortholog DEFB19 demonstrated subfertility in both sexes. DEFB119 is also expressed in sperm and is involved in sperm chemotaxis. Elevated seminal DEFB119 was associated with a decrease in the observed genera, diversity and evenness of bacterial communities, and further distortion of the metacommunities (PMID: 39359400).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DEFB119 antibody 33236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
