验证数据展示
经过测试的应用
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18268-1-AP targets DEFA5 in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag12793 Product name: Recombinant human DEFA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-94 aa of BC069690 Sequence: ERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTGRCATRESLSGVCEISGRLYRLCCR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | defensin, alpha 5, Paneth cell-specific |
| 别名 | DEF5, DEFA5, Defensin 5, Defensin, alpha 5, defensin, alpha 5, Paneth cell, HD 5 |
| 计算分子量 | 94 aa, 10 kDa |
| GenBank蛋白编号 | BC069690 |
| 基因名称 | DEFA5 |
| Gene ID (NCBI) | 1670 |
| RRID | AB_2878526 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01523 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEFA5, also named as DEF5, belongs to the alpha-defensin family. It has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Mature DEFA5 is about 5kd. It has a homodimer form.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DEFA5 antibody 18268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
